OBSERVATION OF NUCLEUS IN ONION PEEL CELLS ( CELL- THE
BASIC UNIT OF LIFE)
Aim: To observe the nucleus in onion peel cells.
Materials required: Onion, slide, methylene blue, cover slip and microscope.
Precautions: Only 1 or 2 drops of stain should be added and extra stain should be
removed.
Procedure:
1. Peeled a membrane from an onion and placed it on a slide.
2. Added 1-2 drops of methylene blue to stain the section.
3. Covered it with a cover slip and left it for 5 minutes.
4. Added drop wise from one side of the cover slip and soaked extra water with
filter paper from the other side.
5. Observed under microscope.
Observations: The rectangular cells are observed with a dense blue colored nucleus
near to the wall.
Details: Onion peel cell is a typical plant cell. It contains rectangular, tightly packed
cells with prominent nucleus. The nucleus is pushed towards the cell wall due to the
presence of a big vacuole.
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